Fig. 7. SUPT5H depletion in MDA-MB-231 inhibits the expression of MAPK pathway proteins. Western blot showing the effects of silencing of SUPT5H on the levels of total and phosphorylated JNK, P38, and c-Jun. An equal amount of cellular protein (50µg) was separated, and β-actin was applied as a control for equal loading (representative blots are shown from two independent experiments).